8PRU
Engineered form of T thermophiles AHIR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.030, 142.720, 126.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.060 - 2.000 |
| Rwork | 0.259 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.449 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.060 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.154 | 1.122 |
| Rmeas | 0.177 | 1.298 |
| Rpim | 0.085 | 0.637 |
| Number of reflections | 184696 | 9269 |
| <I/σ(I)> | 5.6 | 1.5 |
| Completeness [%] | 94.7 | 96.8 |
| Redundancy | 4 | 3.9 |
| CC(1/2) | 0.993 | 0.679 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | PEG 4000 |
