8PRN

E1M, K50A, R52A, D97A, E99A MUTANT OF RH. BLASTICA PORIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-12
Detector	SIEMENS
Spacegroup name	H 3
Unit cell lengths	103.750, 103.750, 124.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.900 - 2.300
R-factor	0.177 *
Rwork	0.177
R-free	0.21500
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1prn
RMSD bond length	0.018
RMSD bond angle	0.036
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000 *	1.970
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.065 *	0.185 *
Number of reflections	20686 *	1565 *
<I/σ(I)>	15.9	2.5
Completeness [%]	93.0 *	72 *
Redundancy	2.9 *	1.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2	20 *	Kreusch, A., (1994) J.Mol.Biol., 243, 891. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		300 (mM)
4	1	drop	n-octyltetraoxyethylene	0.6 (%(w/v))
5	1	drop		3 (mM)
6	1	drop	PEG600	10-18 (%)
7	1	reservoir	PEG600	30-38 (%(w/v))