8PR6
Crystal structure of Corynebacterium glutamicum mycoredoxin-3 at 1.7 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.124, 36.248, 68.719 |
| Unit cell angles | 90.00, 99.01, 90.00 |
Refinement procedure
| Resolution | 32.230 - 1.700 |
| R-factor | 0.21927 |
| Rwork | 0.217 |
| R-free | 0.25679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.400 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 | 0.700 |
| Rmeas | 0.081 | 0.820 |
| Number of reflections | 19317 | 2791 |
| <I/σ(I)> | 9.2 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG 3350, 0.2 M MgCl2, 0.1 M Bis-Tris (pH 5.5) |
