8PQ7
The Potato Late Blight pathogen (Phytophthora infestans) effector protein Pi04134 in complex with potato protein phosphatase type 1c (PP1c).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-25 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.91188 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.492, 57.036, 59.015 |
| Unit cell angles | 84.35, 86.49, 89.85 |
Refinement procedure
| Resolution | 58.620 - 2.100 |
| R-factor | 0.20482 |
| Rwork | 0.202 |
| R-free | 0.25141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5inb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.659 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.620 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.097 | 0.527 |
| Number of reflections | 40927 | 3300 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.997 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M sodium fluoride, 0.1 M BIS-TRIS propose pH 8.5, 20 % PEG3350 |
