8PPW

Structure of human PARK7 in complex with GK16S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-22
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.000
Spacegroup name	P 65 2 2
Unit cell lengths	66.823, 66.823, 177.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.230 - 1.530
R-factor	0.1651
Rwork	0.164
R-free	0.18210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.973
Data reduction software	XDS (VERSION Jan 31, 2020 BUILT=20200417)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.230	1.590
High resolution limit [Å]	1.530	1.530
Rmerge	0.039	1.416
Rmeas	0.041	1.458
Number of reflections	66541	2615
<I/σ(I)>	31.6	2.3
Completeness [%]	99.1
Redundancy	16
CC(1/2)	1.000	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	22.3% (v/v) PEG3350, 230 mM KNO3