8PPS
Dimeric RbdA EAL, in apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.400, 65.720, 172.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.040 - 2.300 |
| Rwork | 0.184 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.551 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP (11.7.03) |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.040 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.059 | 0.918 |
| Number of reflections | 31120 | 1890 |
| <I/σ(I)> | 15.9 | 1.32 |
| Completeness [%] | 93.5 | 78 |
| Redundancy | 4.6 | 3.1 |
| CC(1/2) | 0.999 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 294.15 | 100mM Tris and BICINE pH 9 34% (w/v) ethylene glycol and PEG 8000 mix |
