8POA

Structure of Coxsackievirus A16 (G-10) 2A protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-22
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9212
Spacegroup name	C 1 2 1
Unit cell lengths	86.128, 57.199, 64.631
Unit cell angles	90.00, 95.23, 90.00

Refinement procedure

Resolution	16.090 - 1.600
R-factor	0.187
Rwork	0.186
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.930
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (14-JUN-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.090	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.146	0.704
Rmeas	0.158	0.782
Rpim	0.060	0.334
Number of reflections	41210	4072
<I/σ(I)>	10.43	1.63
Completeness [%]	99.5	98.91
Redundancy	6.7	5.3
CC(1/2)	0.996	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Ammonium Formate 20% PEG 3350