8PO9
Polyethylene oxidation hexamerin PEase Cibeles (XP_026756460) from Galleria mellonella
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2022-07-05 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.265 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.750, 196.580, 224.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.260 - 2.200 |
| R-factor | 0.1737 |
| Rwork | 0.171 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.953 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 209645 | 32642 |
| <I/σ(I)> | 7.21 | 1.32 |
| Completeness [%] | 99.9 | |
| Redundancy | 8.96 | |
| CC(1/2) | 0.990 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 35 % MPD, 100 mM Li2SO4, 100 mM MES pH 6 |
