8PO8
Structure of Escherichia coli HrpA in complex with ADP and oligonucleotide poly(dC)11 forming an i-motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.977770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.260, 105.973, 177.552 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.980 - 2.520 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.747 |
Data reduction software | XDS (BUILT 20190315) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.997 | 2.564 |
High resolution limit [Å] | 2.520 | 2.521 |
Rmerge | 0.106 | 0.852 |
Rmeas | 0.115 | 0.925 |
Rpim | 0.044 | 0.356 |
Number of reflections | 48761 | 2508 |
<I/σ(I)> | 12.3 | 1.9 |
Completeness [%] | 94.0 | 98.7 |
Redundancy | 6.6 | 6.5 |
CC(1/2) | 0.998 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 10% PEG 3350 4% Tacsimate |