8PNX
Crystal structure of D-amino acid aminotransferase from Blastococcus saxobsidens in PMP form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-20 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54184 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 105.437, 105.437, 51.144 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.550 - 1.800 |
R-factor | 0.17795 |
Rwork | 0.176 |
R-free | 0.21079 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.750 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.550 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.111 | 0.462 |
Rmeas | 0.114 | 0.482 |
Rpim | 0.028 | 0.133 |
Total number of observations | 481580 | 21447 |
Number of reflections | 30325 | 1744 |
<I/σ(I)> | 23.2 | 5.5 |
Completeness [%] | 99.2 | |
Redundancy | 15.9 | 12.3 |
CC(1/2) | 0.999 | 0.836 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M Hepes pH 7.5, 4 M NaCl |