8PNX

Crystal structure of D-amino acid aminotransferase from Blastococcus saxobsidens in PMP form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-20
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 31 2 1
Unit cell lengths	105.437, 105.437, 51.144
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.550 - 1.800
R-factor	0.17795
Rwork	0.176
R-free	0.21079
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.750
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.550	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.111	0.462
Rmeas	0.114	0.482
Rpim	0.028	0.133
Total number of observations	481580	21447
Number of reflections	30325	1744
<I/σ(I)>	23.2	5.5
Completeness [%]	99.2
Redundancy	15.9	12.3
CC(1/2)	0.999	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.1 M Hepes pH 7.5, 4 M NaCl