8PNJ
Chorismate mutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.608, 105.344, 55.776 |
| Unit cell angles | 90.00, 112.48, 90.00 |
Refinement procedure
| Resolution | 46.336 - 2.355 |
| Rwork | 0.215 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.451 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.336 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 20939 | 1801 |
| <I/σ(I)> | 4.1 | 1.1 |
| Completeness [%] | 97.3 | 85 |
| Redundancy | 3.1 | |
| CC(1/2) | 0.959 | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 1:1 volume ratio of 5 mg/ml protein in 20 mM TRIS-HCl, pH=8, 150 uM NaCl buffer and 0.1M BTP, pH=8.5 0.2M KSCN 20% PEG3350 |
