8PNA
transcription factor BARHL2 bound to TAATG DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.853, 46.881, 71.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.250 - 1.450 |
| R-factor | 0.1953 |
| Rwork | 0.194 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.513 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.250 | 39.250 | 1.470 |
| High resolution limit [Å] | 1.450 | 7.930 | 1.450 |
| Rmerge | 0.081 | 0.037 | 1.627 |
| Rmeas | 0.086 | 0.040 | 1.907 |
| Rpim | 0.029 | 0.014 | 0.966 |
| Total number of observations | 189240 | 1353 | 3339 |
| Number of reflections | 23842 | 185 | 999 |
| <I/σ(I)> | 13.1 | 42.7 | 0.6 |
| Completeness [%] | 99.1 | 99.4 | 86.2 |
| Redundancy | 7.9 | 7.3 | 3.3 |
| CC(1/2) | 0.999 | 1.000 | 0.288 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium malonate, sodium acetate |
