8PN4
transcription factor BARHL2 bound to DNA sequences
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.426, 134.560, 64.491 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 39.400 - 2.600 |
| R-factor | 0.24507 |
| Rwork | 0.243 |
| R-free | 0.27378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.520 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.224 | 1.827 |
| Rmeas | 0.263 | 2.114 |
| Rpim | 0.135 | 1.055 |
| Total number of observations | 78862 | 9556 |
| Number of reflections | 20553 | 2416 |
| <I/σ(I)> | 5.9 | 1 |
| Completeness [%] | 99.5 | |
| Redundancy | 3.8 | 4 |
| CC(1/2) | 0.985 | 0.285 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium acetate, |
