8PMY
HEV gt3 P domain in complex with glycan-insensitive nAb p60.15
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.190, 122.950, 158.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.650 - 2.406 |
| R-factor | 0.2135 |
| Rwork | 0.212 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.640 | 2.492 |
| High resolution limit [Å] | 2.406 | 2.406 |
| Rmeas | 0.293 | |
| Number of reflections | 21742 | 2122 |
| <I/σ(I)> | 7.43 | |
| Completeness [%] | 99.6 | |
| Redundancy | 13.12 | |
| CC(1/2) | 0.993 | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 11% w/v PEG 8000, 0.1M Zinc acetate and 0.1M MES pH 5.5 |
