8PMV
transcription factor BARHL2 bound to TAAGC DNA sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.885600 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.080, 133.773, 64.990 |
Unit cell angles | 90.00, 92.74, 90.00 |
Refinement procedure
Resolution | 46.630 - 2.100 |
R-factor | 0.25264 |
Rwork | 0.251 |
R-free | 0.27763 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 2.170 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.630 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.120 | 5.172 |
Rmeas | 0.132 | 5.681 |
Rpim | 0.054 | 2.322 |
Total number of observations | 18000 | |
Number of reflections | 38886 | 3154 |
<I/σ(I)> | 7 | 0.3 |
Completeness [%] | 99.7 | |
Redundancy | 5.7 | 5.7 |
CC(1/2) | 0.997 | 0.087 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium malonate, sodium acetate |