8PMV
transcription factor BARHL2 bound to TAAGC DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.885600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.080, 133.773, 64.990 |
| Unit cell angles | 90.00, 92.74, 90.00 |
Refinement procedure
| Resolution | 46.630 - 2.100 |
| R-factor | 0.25264 |
| Rwork | 0.251 |
| R-free | 0.27763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.630 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 5.172 |
| Rmeas | 0.132 | 5.681 |
| Rpim | 0.054 | 2.322 |
| Total number of observations | 18000 | |
| Number of reflections | 38886 | 3154 |
| <I/σ(I)> | 7 | 0.3 |
| Completeness [%] | 99.7 | |
| Redundancy | 5.7 | 5.7 |
| CC(1/2) | 0.997 | 0.087 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium malonate, sodium acetate |
