8PMM

Structure of Nal1 protein, allele SPIKE from japonica rice, construct 31-458

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978690
Spacegroup name	C 2 2 21
Unit cell lengths	89.967, 182.917, 172.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.840 - 1.750
R-factor	0.2019
Rwork	0.200
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.974
Data reduction software	XDS (BUILT 20200417)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.459	1.910
High resolution limit [Å]	1.748	1.748
Rmerge	0.077	1.339
Rmeas	0.080	1.391
Rpim	0.022	0.374
Number of reflections	107716	5386
<I/σ(I)>	18.1	1.9
Completeness [%]	94.9	61.7
Redundancy	13.5	13.7
CC(1/2)	0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	4% tacsimate 12% PEG 3350