8PMI
Structure of Nal1 indica cultivar IR64, construct 36-458 in presence of peptide from FZP protein
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 174.276, 189.186, 89.922 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.500 - 2.830 |
| R-factor | 0.2093 |
| Rwork | 0.206 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.553 |
| Data reduction software | XDS (BUILT 20220820) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.593 | 3.132 |
| High resolution limit [Å] | 2.826 | 2.826 |
| Rmerge | 0.245 | 2.090 |
| Rmeas | 0.254 | 2.178 |
| Rpim | 0.069 | 0.608 |
| Number of reflections | 50605 | 2530 |
| <I/σ(I)> | 9.2 | 1.6 |
| Completeness [%] | 95.1 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.995 | 0.588 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.05M MES 12% methanol 4% PEG 2000 |
