8PMG

Structure of Nal1 indica cultivar IR64, construct 36-458

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980110
Spacegroup name	P 21 21 2
Unit cell lengths	173.410, 191.031, 90.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.600 - 3.290
R-factor	0.2141
Rwork	0.211
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.642
Data reduction software	XDS (BUILT 20200417)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.516	3.468
High resolution limit [Å]	3.290	3.292
Rmerge	0.186	1.362
Rmeas	0.205	1.481
Rpim	0.084	0.573
Number of reflections	33531	1678
<I/σ(I)>	7.2	1.6
Completeness [%]	88.2	83.7
Redundancy	5.8	6.5
CC(1/2)	0.994	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.05M MES 12% methanol 4% PEG 2000