8PMC
transcription factor BARHL2 bound to TAATT DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.595, 123.148, 62.198 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 39.520 - 1.850 |
| R-factor | 0.2196 |
| Rwork | 0.218 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.550 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.700 | 43.700 | 1.890 |
| High resolution limit [Å] | 1.850 | 9.070 | 1.850 |
| Rmerge | 0.080 | 0.024 | 3.019 |
| Rmeas | 0.093 | 0.029 | 3.513 |
| Rpim | 0.047 | 0.015 | 1.774 |
| Total number of observations | 190158 | 1560 | 11300 |
| Number of reflections | 50287 | 443 | 3039 |
| <I/σ(I)> | 8.5 | 37.9 | 0.4 |
| Completeness [%] | 99.5 | 97.3 | 96.2 |
| Redundancy | 3.8 | 3.5 | 3.7 |
| CC(1/2) | 0.998 | 0.998 | 0.154 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium malonate, sodium acetate |
