8PM5
transcription factor BARHL2 bound to TAAAT DNA sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.015, 134.298, 65.625 |
Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
Resolution | 46.950 - 2.400 |
R-factor | 0.23118 |
Rwork | 0.229 |
R-free | 0.28042 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 2.111 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.950 | 2.410 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.154 | 2.965 |
Rmeas | 0.167 | 3.220 |
Rpim | 0.063 | 1.233 |
Total number of observations | 13576 | |
Number of reflections | 27918 | 2056 |
<I/σ(I)> | 8.8 | 0.7 |
Completeness [%] | 96.6 | |
Redundancy | 7.2 | 6.6 |
CC(1/2) | 0.996 | 0.179 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium malonate, sodium acetate |