8PKY

Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148V variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	53.799, 109.386, 39.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.753 - 1.400
Rwork	0.142
R-free	0.18550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.390	1.420
High resolution limit [Å]	1.400	1.400
Number of reflections	45300	2160
<I/σ(I)>	18.3
Completeness [%]	98.0
Redundancy	14.1
CC(1/2)	1.000	0.412

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Magnesium chloride hexahydrate 0.1 M TRIS hydrochloride pH 8.5 30% w/v Polyethylene glycol 4,000