8PJR

Crystal structure of the SAKe6AC-LB protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-30
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.867023
Spacegroup name	P 1 21 1
Unit cell lengths	34.730, 47.556, 74.486
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.080 - 1.710
R-factor	0.1642
Rwork	0.163
R-free	0.18250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.056
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.080	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.068	0.189
Rmeas	0.075	0.204
Rpim	0.030	0.075
Total number of observations	171256	9812
Number of reflections	25559	1364
<I/σ(I)>	18.9	11.2
Completeness [%]	96.9
Redundancy	6.7	7.2
CC(1/2)	0.998	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	0.2 M Magnesium formate pH 5.9, 20% PEG 3350