8PJQ
Crystal structure of the SAKe2BE protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.867023 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 44.226, 44.226, 70.274 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.140 - 1.150 |
| R-factor | 0.1793 |
| Rwork | 0.179 |
| R-free | 0.18450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.838 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.057 | 0.757 |
| Rmeas | 0.059 | 0.777 |
| Rpim | 0.013 | 0.174 |
| Total number of observations | 568939 | 28623 |
| Number of reflections | 28974 | 1444 |
| <I/σ(I)> | 24.1 | 4.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 19.6 | 19.8 |
| CC(1/2) | 0.998 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | 0.018 M Calcium chloride, 0.09 M Sodium acetate pH 4.6, 27% (v/v) MPD, 10% (v/v) glycerol |
