8PJD

Crystal Structure of Mus musculus Protein Arginine Methyltransferase 2 94-445

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-04
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9677
Spacegroup name	C 2 2 21
Unit cell lengths	65.052, 115.980, 133.718
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.250 - 1.600
R-factor	0.1723
Rwork	0.171
R-free	0.18910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.005
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.21rc1_4958: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.250	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.075	2.384
Rmeas	0.078	2.527
Rpim	0.021	0.808
Number of reflections	65279	2584
<I/σ(I)>	17.7	0.8
Completeness [%]	97.5	77.85
Redundancy	7	9.2
CC(1/2)	0.999	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	1.3M AcONa pH5.3