8PJC

Crystal structure of human insulin desB30 precursor with an Alanine-Alanine-Lysine C-peptide in dimer (T2) conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-01-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	I 21 3
Unit cell lengths	77.874, 77.874, 77.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.360 - 2.140
R-factor	0.1752
Rwork	0.174
R-free	0.20700
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	0.746
Data reduction software	HKL-2000 (0.93)
Data scaling software	HKL-2000 (0.93)
Phasing software	REFMAC
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.220
High resolution limit [Å]	2.140	2.140
Rmerge	0.053	0.256
Number of reflections	4325	1073
<I/σ(I)>	14.4
Completeness [%]	96.5	89.1
Redundancy	2.97	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	TBD