8PIH

Structure of Api m1 in complex with two nanobodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	133.960, 51.330, 73.250
Unit cell angles	90.00, 120.78, 90.00

Refinement procedure

Resolution	42.020 - 1.760
R-factor	0.1725
Rwork	0.171
R-free	0.20190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.020	1.823
High resolution limit [Å]	1.760	1.760
Rmerge	0.078
Number of reflections	42066	4105
<I/σ(I)>	12.71
Completeness [%]	98.6	97.34
Redundancy	4.4	4.6
CC(1/2)	0.999	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.2 M NaCl, 0.1 M Phosphate/citrate pH 4.2, 20% w/v polyethylene glycol (PEG) 8000