8PIH
Structure of Api m1 in complex with two nanobodies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.960, 51.330, 73.250 |
Unit cell angles | 90.00, 120.78, 90.00 |
Refinement procedure
Resolution | 42.020 - 1.760 |
R-factor | 0.1725 |
Rwork | 0.171 |
R-free | 0.20190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.989 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.020 | 1.823 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.078 | |
Number of reflections | 42066 | 4105 |
<I/σ(I)> | 12.71 | |
Completeness [%] | 98.6 | 97.34 |
Redundancy | 4.4 | 4.6 |
CC(1/2) | 0.999 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 292 | 0.2 M NaCl, 0.1 M Phosphate/citrate pH 4.2, 20% w/v polyethylene glycol (PEG) 8000 |