8PIF
Fragment 12 in complex with KLHDC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000018 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.261, 88.308, 88.922 |
| Unit cell angles | 90.00, 104.51, 90.00 |
Refinement procedure
| Resolution | 35.080 - 1.782 |
| R-factor | 0.1833 |
| Rwork | 0.182 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC (1.1.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.642 | 1.958 |
| High resolution limit [Å] | 1.782 | 1.782 |
| Rmerge | 0.099 | 1.262 |
| Number of reflections | 43588 | 2179 |
| <I/σ(I)> | 11.3 | 1.6 |
| Completeness [%] | 69.0 | 14.1 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 23%PEG3350 100mM HEPES pH 7.0 200mM MgCl2 |
