8PI9

DNA binding domain of HNF-1A bound to P2-HNF4A promoter DNA variant (P2 -181G>A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	49.008, 53.065, 202.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.640 - 2.800
R-factor	0.2368
Rwork	0.234
R-free	0.29340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.698
Data reduction software	XDS
Data scaling software	XSCALE (Jan 10, 2022)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.640	2.920
High resolution limit [Å]	2.800	2.800
Rmeas	0.116
Number of reflections	13600	994
<I/σ(I)>	9.77	0.43
Completeness [%]	99.1	99.6
Redundancy	7.4	7.5
CC(1/2)	0.997	0.307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	0.2 M Ca acetate, 0.1 M Na cacodylate, pH 6.5, 40% v/v PEG300