8PI9
DNA binding domain of HNF-1A bound to P2-HNF4A promoter DNA variant (P2 -181G>A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.008, 53.065, 202.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.640 - 2.800 |
R-factor | 0.2368 |
Rwork | 0.234 |
R-free | 0.29340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.698 |
Data reduction software | XDS |
Data scaling software | XSCALE (Jan 10, 2022) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.640 | 2.920 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmeas | 0.116 | |
Number of reflections | 13600 | 994 |
<I/σ(I)> | 9.77 | 0.43 |
Completeness [%] | 99.1 | 99.6 |
Redundancy | 7.4 | 7.5 |
CC(1/2) | 0.997 | 0.307 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.2 M Ca acetate, 0.1 M Na cacodylate, pH 6.5, 40% v/v PEG300 |