8PI7

DNA binding domain of HNF-1A bound to P2-HNF4A promoter DNA variant (P2 -169C>T)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-20
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	49.456, 52.506, 210.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.140 - 3.200
R-factor	0.2267
Rwork	0.221
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.425
Data reduction software	XDS
Data scaling software	XSCALE (Jan 10, 2022)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.140	3.290
High resolution limit [Å]	3.200	3.200
Rmeas	0.160
Number of reflections	9655	729
<I/σ(I)>	10.86	1.34
Completeness [%]	99.6	99.7
Redundancy	7.2	7.6
CC(1/2)	0.997	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	10% w/v PEG 1000