8PI7
DNA binding domain of HNF-1A bound to P2-HNF4A promoter DNA variant (P2 -169C>T)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-20 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.456, 52.506, 210.278 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.140 - 3.200 |
R-factor | 0.2267 |
Rwork | 0.221 |
R-free | 0.28040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.425 |
Data reduction software | XDS |
Data scaling software | XSCALE (Jan 10, 2022) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.140 | 3.290 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmeas | 0.160 | |
Number of reflections | 9655 | 729 |
<I/σ(I)> | 10.86 | 1.34 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 7.2 | 7.6 |
CC(1/2) | 0.997 | 0.572 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 10% w/v PEG 1000 |