8PHX

Receiver Domain of the Hybrid Histidine Kinase Sln1 of Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 2 21 21
Unit cell lengths	65.163, 96.272, 97.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.200 - 1.500
R-factor	0.19435
Rwork	0.193
R-free	0.21521
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.360
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.280	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.058	0.995
Rmeas	0.062	1.069
Rpim	0.023	0.386
Total number of observations	723181	35636
Number of reflections	98531	4803
<I/σ(I)>	17.3	2.2
Completeness [%]	100.0
Redundancy	7.3	7.4
CC(1/2)	1.000	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	30 % w/v PEG 4000 0.1 M Tris-HCl pH 8.5 0.2 M Magnesium Chloride