8PHX
Receiver Domain of the Hybrid Histidine Kinase Sln1 of Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 65.163, 96.272, 97.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.200 - 1.500 |
| R-factor | 0.19435 |
| Rwork | 0.193 |
| R-free | 0.21521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.360 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.280 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.058 | 0.995 |
| Rmeas | 0.062 | 1.069 |
| Rpim | 0.023 | 0.386 |
| Total number of observations | 723181 | 35636 |
| Number of reflections | 98531 | 4803 |
| <I/σ(I)> | 17.3 | 2.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.3 | 7.4 |
| CC(1/2) | 1.000 | 0.749 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 30 % w/v PEG 4000 0.1 M Tris-HCl pH 8.5 0.2 M Magnesium Chloride |
