8PHN
Receiver domain from hybrid Histidine Kinase 3 from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 154.268, 154.268, 154.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.070 - 1.900 |
| R-factor | 0.21169 |
| Rwork | 0.211 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.070 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.088 | 1.245 |
| Rmeas | 0.090 | 1.275 |
| Rpim | 0.020 | 0.272 |
| Total number of observations | 275556 | 17418 |
| Number of reflections | 12954 | 803 |
| <I/σ(I)> | 22.3 | 3 |
| Completeness [%] | 100.0 | |
| Redundancy | 21.3 | 21.7 |
| CC(1/2) | 1.000 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 2M ammonium sulphate 0.1 M Tris pH 8.5 |
