8PHB
Crystal structure of apo Cami1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-07 |
Detector | DECTRIS EIGER2 R 1M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.238, 90.428, 65.616 |
Unit cell angles | 90.00, 99.56, 90.00 |
Refinement procedure
Resolution | 64.710 - 1.700 |
R-factor | 0.1887 |
Rwork | 0.186 |
R-free | 0.21470 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.830 |
Data reduction software | XDS (Mar 15, 2019) |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHELX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.710 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.042 | 0.669 |
Rmeas | 0.049 | 0.784 |
Rpim | 0.018 | 0.292 |
Total number of observations | 578268 | 83153 |
Number of reflections | 81871 | 11877 |
<I/σ(I)> | 17.7 | 2.5 |
Completeness [%] | 99.3 | |
Redundancy | 7.1 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.02 M carboxylic acids, 0.1 M MOPS/HEPES pH 7.5, 10% PEG 20K, 10% PEG400 |