8PFW
X-ray structure of the adduct formed upon reaction of Lysozyme with K2[Ru2(DAniF)(CO3)3] in condition A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.810, 77.810, 37.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.020 - 1.120 |
| Rwork | 0.201 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 3.691 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.020 | 1.140 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.054 | 1.324 |
| Number of reflections | 44515 | 2171 |
| <I/σ(I)> | 27.2 | 2.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 11 | |
| CC(1/2) | 0.999 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 20% ethylene glycol, 0.6 M NaNO3, 0.1 M NaOAc pH 4.5 |
