8PFO
Crystal structure of WRN helicase domain in complex with HRO761
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.179, 87.418, 133.776 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.850 - 1.900 |
| R-factor | 0.2087 |
| Rwork | 0.207 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.890 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.200 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.112 | 2.077 |
| Rmeas | 0.120 | 2.241 |
| Number of reflections | 33000 | 1650 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 84.4 | 29.4 |
| Redundancy | 7.5 | 8 |
| CC(1/2) | 0.997 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25.5 % PEG 4000 0.17 M ammonium sulfate 15 % glycerol |
