8PFD

Crystal structure of binary complex between Aster yellows witches'-broom phytoplasma effector SAP05 and the von Willebrand Factor Type A domain of the proteasomal ubiquitin receptor Rpn10 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	42.391, 68.599, 49.851
Unit cell angles	90.00, 92.78, 90.00

Refinement procedure

Resolution	49.840 - 2.170
R-factor	0.20035
Rwork	0.198
R-free	0.25225
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.442
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.600	2.240
High resolution limit [Å]	2.170	2.170
Rmerge	0.118	2.599
Rmeas	0.122	2.700
Rpim	0.033	0.729
Total number of observations	210355	18117
Number of reflections	15199	1337
<I/σ(I)>	10.9	1
Completeness [%]	100.0
Redundancy	13.8	13.6
CC(1/2)	0.999	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	NULL