8PFD
Crystal structure of binary complex between Aster yellows witches'-broom phytoplasma effector SAP05 and the von Willebrand Factor Type A domain of the proteasomal ubiquitin receptor Rpn10 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-25 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.391, 68.599, 49.851 |
| Unit cell angles | 90.00, 92.78, 90.00 |
Refinement procedure
| Resolution | 49.840 - 2.170 |
| R-factor | 0.20035 |
| Rwork | 0.198 |
| R-free | 0.25225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.442 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.600 | 2.240 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.118 | 2.599 |
| Rmeas | 0.122 | 2.700 |
| Rpim | 0.033 | 0.729 |
| Total number of observations | 210355 | 18117 |
| Number of reflections | 15199 | 1337 |
| <I/σ(I)> | 10.9 | 1 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.8 | 13.6 |
| CC(1/2) | 0.999 | 0.436 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | NULL |
