8PEL

Structure of C. thermophilum RNA exosome core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99994
Spacegroup name	P 21 21 21
Unit cell lengths	100.525, 148.385, 195.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.900 - 3.810
R-factor	0.2456
Rwork	0.243
R-free	0.29250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.692
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	XSCALE (Jan 10, 2022)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	3.946
High resolution limit [Å]	3.810	3.810
Number of reflections	29143	2793
<I/σ(I)>	5.01	1.19
Completeness [%]	99.6	97.14
Redundancy	13.5	13.5
CC(1/2)	0.984	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.2M Ammonium Sulphate 0.1M Sodium Acetate pH 5.5 10% PEG MME 2000