8PEF

Crystal structure of SLF1 Ankyrin repeat domain in complex with H4 tail (K20me0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	C 2 2 21
Unit cell lengths	37.680, 50.940, 139.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 1.280
R-factor	0.1955
Rwork	0.194
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.770
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	1.326
High resolution limit [Å]	1.280	1.280
Number of reflections	35141	3459
<I/σ(I)>	10.02
Completeness [%]	100.0
Redundancy	11.4
CC(1/2)	0.990	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1M Sodium malonate dibasic monohydrate 0.1M Hepes pH 7 30% (w/v) Poly(acrylic acid sodium salt) 2100