8PE1
Crystal structure of Gel4 in complex with Nanobody 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.388, 86.330, 178.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.010 - 1.900 |
R-factor | 0.21726 |
Rwork | 0.215 |
R-free | 0.26446 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w62 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.601 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.010 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.182 | 1.588 |
Number of reflections | 104001 | 15033 |
<I/σ(I)> | 6 | 1 |
Completeness [%] | 99.6 | |
Redundancy | 5.5 | |
CC(1/2) | 0.994 | 0.537 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Halogens, buffer system 3, P500MME P20K |