8PE1
Crystal structure of Gel4 in complex with Nanobody 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.388, 86.330, 178.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.010 - 1.900 |
| R-factor | 0.21726 |
| Rwork | 0.215 |
| R-free | 0.26446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w62 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.601 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.010 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.182 | 1.588 |
| Number of reflections | 104001 | 15033 |
| <I/σ(I)> | 6 | 1 |
| Completeness [%] | 99.6 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.994 | 0.537 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Halogens, buffer system 3, P500MME P20K |
