8PE0
X-ray structure of the Thermus thermophilus K167L mutant of the PilF-GSPIIB domain in the c-di-GMP bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | H 3 |
Unit cell lengths | 108.434, 108.434, 88.125 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.440 - 1.900 |
R-factor | 0.1808 |
Rwork | 0.179 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.260 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.261 | 1.968 |
High resolution limit [Å] | 1.760 | 1.900 |
Rmerge | 0.072 | 1.713 |
Rmeas | 0.075 | |
Rpim | 0.023 | 0.552 |
Number of reflections | 30444 | 3034 |
<I/σ(I)> | 15.02 | 1.23 |
Completeness [%] | 100.0 | |
Redundancy | 10.4 | |
CC(1/2) | 0.999 | 0.635 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 1.5 M LiSO4 0.1 M Na-Acetate pH 6.0 |