8PCW
Structure of Csm6' from Streptococcus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2019-10-14 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.646, 104.138, 144.478 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.520 - 3.540 |
| R-factor | 0.34359 |
| Rwork | 0.341 |
| R-free | 0.38745 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.226 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.520 | 3.630 |
| High resolution limit [Å] | 3.540 | 3.540 |
| Rmerge | 0.090 | 1.340 |
| Number of reflections | 15935 | 1035 |
| <I/σ(I)> | 9.4 | 1.3 |
| Completeness [%] | 95.2 | 85.5 |
| Redundancy | 3.8 | 3.4 |
| CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.95 M sodium citrate, 0.18 M sodium bromide and 0.1 M HEPES, pH 8 |
