8PC4

MEMBRANE TARGET COMPLEX 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	94.389, 94.389, 442.325
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.750 - 2.850
R-factor	0.2669
Rwork	0.265
R-free	0.30110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.261
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.470	3.020
High resolution limit [Å]	2.660	2.860
Rmerge	0.142	1.500
Number of reflections	35073	4539
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Lithium Sulfate