8PBW
Histidine-containing phosphotransfer protein from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-27 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.885, 149.334, 65.365 |
| Unit cell angles | 90.00, 101.02, 90.00 |
Refinement procedure
| Resolution | 74.780 - 2.400 |
| R-factor | 0.23115 |
| Rwork | 0.229 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.780 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.091 | 0.570 |
| Rmeas | 0.098 | 0.623 |
| Rpim | 0.038 | 0.248 |
| Total number of observations | 22834 | |
| Number of reflections | 35171 | 3660 |
| <I/σ(I)> | 10.6 | 2.7 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 | 6.2 |
| CC(1/2) | 0.994 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 1.5 M Sodium Citrate pH 6.5 |
