8PAE
Structure of the ectodomain of Atypical Porcine Pestivirus E2 at 1.2A resolution
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | H 3 |
| Unit cell lengths | 101.124, 101.124, 43.124 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.260 - 1.200 |
| R-factor | 0.1518 |
| Rwork | 0.150 |
| R-free | 0.17970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.136 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.260 | 1.243 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.032 | 0.597 |
| Rmeas | 0.036 | 0.753 |
| Rpim | 0.016 | 0.451 |
| Number of reflections | 93856 | 2865 |
| <I/σ(I)> | 18.37 | |
| Completeness [%] | 91.3 | 56.09 |
| Redundancy | 4.7 | 2.4 |
| CC(1/2) | 0.999 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 0.1 M Bis Tris pH 7.8, 0.05 M sodium citrate, 0.05 M magnesium chloride, 25% PEG Smear High |
