8PA0

cvHsp (HspB7) C131S alpha-crystallin domain - filamin C (FLNC) domain 24 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.976
Spacegroup name	P 32 2 1
Unit cell lengths	94.129, 94.129, 51.597
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.060 - 2.850
R-factor	0.2276
Rwork	0.224
R-free	0.29090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.516
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.060	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.288	2.487
Number of reflections	6383	316
<I/σ(I)>	6.1	1.9
Completeness [%]	100.0	100
Redundancy	19.2	19.83
CC(1/2)	0.995	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 mM HEPES and 20% w/v PEG 8000, pH 7.0