8P9O

PCNA from Chaetomium thermophilum in complex with PolD3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2023-02-20
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	55.840, 56.391, 62.860
Unit cell angles	90.93, 90.55, 99.48

Refinement procedure

Resolution	27.570 - 2.450
R-factor	0.2144
Rwork	0.213
R-free	0.24943
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.962
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.570	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.091	0.255
Rmeas	0.113	0.324
Rpim	0.067	0.197
Total number of observations	66712	6953
Number of reflections	25893	2840
<I/σ(I)>	5.7	2.8
Completeness [%]	93.2
Redundancy	2.6	2.4
CC(1/2)	0.988	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.1 M Phosphate/citrate, pH4.2, 40% v/v PEG300