8P9M

Hexameric Hfq from Chromobacterium haemolyticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-30
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	58.505, 71.653, 96.652
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.330 - 2.000
R-factor	0.2248
Rwork	0.222
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.927
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.330	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.068	0.760
Rmeas	0.096	1.070
Rpim	0.068	0.760
Number of reflections	27718	5300
<I/σ(I)>	8.19	0.92
Completeness [%]	98.4	99.71
Redundancy	1.9	1.9
CC(1/2)	0.997	0.579

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	15% PEG4000, 50 mM Tris-HCl, pH 7.5, 150 mM KCl, 20 mM MgCl2