8P8T
Ex vivo Ym2 crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000089224 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.261, 60.205, 60.173 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 16.780 - 1.710 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS (VERSION Jan 26, 2018 BUILT=20180808) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.010 | 1.810 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.177 | 1.364 |
| Number of reflections | 39074 | 6228 |
| <I/σ(I)> | 6.59 | 0.96 |
| Completeness [%] | 97.7 | |
| Redundancy | 3.47 | |
| CC(1/2) | 0.992 | 0.358 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 310 | Single ex vivo Ym2 crystals were harvested from the BAL fluid of transgenic C57BL/6J-Tg(CAG-Gal10)Bla mice and cryoprotected by a brief soak in PBS + 35% (v/v) glycerol. |
