8P8P
Crystal structure of human Histidine Triad Nucleotide-Binding Protein 1 in complex with 5'-O-[(3-Indolyl)-1-Ethyl]Carbamoyl Ethenoadenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-07 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.725, 46.552, 64.101 |
| Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
| Resolution | 19.220 - 1.900 |
| Rwork | 0.154 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.461 |
| Data reduction software | CrysalisPro (1.171.42.80a) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.220 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.331 |
| Rmeas | 0.116 | 0.400 |
| Rpim | 0.057 | 0.220 |
| Number of reflections | 18317 | 1200 |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 3.9 | 2.9 |
| CC(1/2) | 0.994 | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 10% w/v PEG4000, 0.1 M sodium cacodylate pH 6.0 |
