8P8H

Crystal structure of HHD2 domain of hRTEL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	65.590, 60.680, 79.530
Unit cell angles	90.00, 96.13, 90.00

Refinement procedure

Resolution	48.140 - 2.300
Rwork	0.214
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.887
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	27255	4294
<I/σ(I)>	10.53	1.99
Completeness [%]	97.9	96.2
Redundancy	6
CC(1/2)	0.997	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M CHES pH 9.5, 30% PEG 3000