8P84
X-ray structure of Thermoanaerobacterales bacterium monoamine oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.931, 94.167, 127.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.130 - 1.500 |
| R-factor | 0.16028 |
| Rwork | 0.159 |
| R-free | 0.18549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.831 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.130 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 1.158 |
| Rpim | 0.033 | 0.709 |
| Number of reflections | 131032 | 6419 |
| <I/σ(I)> | 17.6 | 1.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.999 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% PEG4000, 100 mM tris buffer pH 8.5, 200 mM magnesium chloride |
